primary antibody against synapsin  (Developmental Studies Hybridoma Bank)

Journal: bioRxiv

Article Title: Synaptic phosphoproteome modifications and cortical circuit dysfunction are linked to the early-stage progression of alpha-synuclein aggregation

doi: 10.1101/2025.01.24.634820

Figure Lengend Snippet: (A) Image of Western blot loaded with homogenates prepared from rat sensorimotor cortex 3 months after intrastriatal injection with aSyn PFFs or monomer. The blot was probed with antibodies specific for the synaptic proteins synaptobrevin, syntaxin, and synapsin-1 or the cytoskeletal proteins tau, β-tubulin, and β-actin (n=4 biological replicates). (B) Graph showing band intensities determined for the Western blot in panel A (mean ± SD).

Article Snippet: After transfer, membranes were blocked in LiCor blocking buffer for 60 min at 22°C and then incubated overnight at 4°C with primary antibodies against synapsin (cat. No. D1265, Cell Signaling Technology, 1:1000), syntaxin (cat. no. Ab188583, Abcam, 1:1000), synaptobrevin (cat. no. 1933-SYB, Antibodies Incorporated, 1:1000), tau (cat. No MAB3420, Millipore Sigma, 1:500), actin (A5441, Sigma-Aldrich, 1:2000), or tubulin (cat. no. 15115, Cell Signaling Technology, 1:1000).

Techniques: Western Blot, Injection